TY - JOUR
T1 - Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA
AU - Rodríguez-Tanty, Ch
AU - Pérez, R.
AU - Miranda, J.
AU - Vélez-Castro, H.
AU - Rosado, A.
AU - Macías, A.
AU - Vidal-Alles, V.
AU - Araña, M. J.
AU - Souto, N. Pérez
AU - Higginson-Clarke, D.
AU - Riverón, A. M.
PY - 2001
Y1 - 2001
N2 - We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.
AB - We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.
UR - http://www.scopus.com/inward/record.url?scp=0034866333&partnerID=8YFLogxK
U2 - 10.1081/NCN-100105240
DO - 10.1081/NCN-100105240
M3 - Article
C2 - 11554538
AN - SCOPUS:0034866333
SN - 1525-7770
VL - 20
SP - 1449
EP - 1461
JO - Nucleosides, Nucleotides and Nucleic Acids
JF - Nucleosides, Nucleotides and Nucleic Acids
IS - 8
ER -