Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Mario Culebras; Cristian J. Grande; Fernando G. Torres; Omar P. Troncoso; Clara M. Gomez; M. Carmen Bañó

doi:10.1080/00914037.2014.958829

Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Mario Culebras, Cristian J. Grande, Fernando G. Torres, Omar P. Troncoso, Clara M. Gomez, M. Carmen Bañó

Sección de Ingeniería Mecánica

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17 Citas (Scopus)

Resumen

Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

Idioma original	Inglés
Páginas (desde-hasta)	411-415
Número de páginas	5
Publicación	International Journal of Polymeric Materials and Polymeric Biomaterials
Volumen	64
N.º	8
DOI	https://doi.org/10.1080/00914037.2014.958829
Estado	Publicada - 12 set. 2015

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title = "Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine",

abstract = "Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.",

keywords = "Bacterial cellulose, cell growth, collagen, poly-L-lysine, tissue engeneering",

author = "Mario Culebras and Grande, {Cristian J.} and Torres, {Fernando G.} and Troncoso, {Omar P.} and Gomez, {Clara M.} and Ba{\~n}{\'o}, {M. Carmen}",

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Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine. / Culebras, Mario; Grande, Cristian J.; Torres, Fernando G. et al.
En: International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 64, N.º 8, 12.09.2015, p. 411-415.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

AU - Culebras, Mario

AU - Grande, Cristian J.

AU - Torres, Fernando G.

AU - Troncoso, Omar P.

AU - Gomez, Clara M.

AU - Bañó, M. Carmen

PY - 2015/9/12

Y1 - 2015/9/12

N2 - Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

AB - Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

KW - Bacterial cellulose

KW - cell growth

KW - collagen

KW - poly-L-lysine

KW - tissue engeneering

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U2 - 10.1080/00914037.2014.958829

DO - 10.1080/00914037.2014.958829

M3 - Article

AN - SCOPUS:84926042657

SN - 0091-4037

VL - 64

SP - 411

EP - 415

JO - International Journal of Polymeric Materials and Polymeric Biomaterials

JF - International Journal of Polymeric Materials and Polymeric Biomaterials

IS - 8

ER -

Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

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