TY - JOUR
T1 - Micromachined separation chips with post-column enzymatic reactions of "class" enzymes and end-column electrochemical detection
T2 - Assays of amino acids
AU - Wang, Joseph
AU - Chatrathi, Madhu Prakash
AU - Ibáñez, Alfredo
AU - Escarpa, Alberto
PY - 2002
Y1 - 2002
N2 - Microchip devices integrating eletrophoretic separations, post-column enzymatic derivatization reactions, and amperometric detection, have been developed. The performance of the new integrated microfabricated chip system is demonstrated for on-chip assay of amino acids based on their electrophoretic separation, post-column reaction with amino-acid oxidase and amperometric detection of the hydrogen peroxide product. Factors influencing the response are examined and optimized, and the analytical performance is characterized. The concept can be extended to different target analytes based on the post-column reactions of other "class" enzymes.
AB - Microchip devices integrating eletrophoretic separations, post-column enzymatic derivatization reactions, and amperometric detection, have been developed. The performance of the new integrated microfabricated chip system is demonstrated for on-chip assay of amino acids based on their electrophoretic separation, post-column reaction with amino-acid oxidase and amperometric detection of the hydrogen peroxide product. Factors influencing the response are examined and optimized, and the analytical performance is characterized. The concept can be extended to different target analytes based on the post-column reactions of other "class" enzymes.
KW - Amino acids
KW - Amperometric detection
KW - Capillary electrophoresis
KW - Derivatization
KW - Microchip
UR - http://www.scopus.com/inward/record.url?scp=0036227408&partnerID=8YFLogxK
U2 - 10.1002/1521-4109(200203)14:6<400::AID-ELAN400>3.0.CO;2-W
DO - 10.1002/1521-4109(200203)14:6<400::AID-ELAN400>3.0.CO;2-W
M3 - Article
AN - SCOPUS:0036227408
SN - 1040-0397
VL - 14
SP - 400
EP - 404
JO - Electroanalysis
JF - Electroanalysis
IS - 6
ER -