Mass spectrometry-based metabolomics of single yeast cells

Alfredo J. Ibáneza, Stephan R. Fagerer, Anna Mareike Schmidt, Pawel L. Urban, Konstantins Jefimovs, Philipp Geiger, Reinhard Dechant, Matthias Heinemann, Renato Zenobi

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

193 Citas (Scopus)

Resumen

Single-cell level measurements are necessary to characterize the intrinsic biological variability in a population of cells. In this study, we demonstrate that, with the microarrays for mass spectrometry platform, we are able to observe this variability. We monitor environmentally (2-deoxy-D-glucose) and genetically (?PFK2) perturbed Saccharomyces cerevisiae cells at the single-cell, few-cell, and population levels. Correlation plots between metabolites from the glycolytic pathway, as well as with the observed ATP/ADP ratio as a measure of cellular energy charge, give biological insight that is not accessible from population-level metabolomic data.

Idioma originalInglés
Páginas (desde-hasta)8790-8794
Número de páginas5
PublicaciónProceedings of the National Academy of Sciences of the United States of America
Volumen110
N.º22
DOI
EstadoPublicada - 28 may. 2013
Publicado de forma externa

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