Invivo tumor targeting via nanoparticle-mediated therapeutic siRNA coupled to inflammatory response in lung cancer mouse models

João Conde, Furong Tian, Yulán Hernández, Chenchen Bao, Daxiang Cui, Klaus Peter Janssen, M. Ricardo Ibarra, Pedro V. Baptista, Tobias Stoeger, Jesús M. de la Fuente

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

133 Citas (Scopus)

Resumen

Up to now, functionalized gold nanoparticles have been optimized as an effective intracellular invitro delivery vehicle for siRNAs to interfere with the expression of specific genes by selective targeting, and provide protection against nucleases. Few examples however of suchlike invivo applications have been described so far. In this study, we report the use of siRNA/RGD gold nanoparticles capable of targeting tumor cells in a lung cancer syngeneic orthotopic murine model. Therapeutic RGD-nanoparticle treatment resulted in successful targeting evident from significant c-myc oncogene down-regulation followed by tumor growth inhibition and prolonged survival of lung tumor bearing mice, possibly via αvβ3 integrin interaction. Our results suggest that RGD gold nanoparticles-mediated delivery of siRNA by intratracheal instillation in mice leads to successful suppression of tumor cell proliferation and respective tumor size reduction. These results reiterate the capability of functionalized gold nanoparticles for targeted delivery of siRNA to cancer cells towards effective silencing of the specific target oncogene. What is more, we demonstrate that the gold-nanoconjugates trigger a complex inflammatory and immune response that might promote the therapeutic effect of the RNAi to reduce tumor size with low doses of siRNA.

Idioma originalInglés
Páginas (desde-hasta)7744-7753
Número de páginas10
PublicaciónBiomaterials
Volumen34
N.º31
DOI
EstadoPublicada - oct. 2013
Publicado de forma externa

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