Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

A. M. Vazquez; M. Alfonso; B. Lanne; K. A. Karlsson; A. Carr; O. Barroso; L. E. Fernandez; E. Rengifo; M. E. Lanio; C. Alvarez; J. Zeuthen; R. Perez

doi:10.1089/hyb.1995.14.551

Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

A. M. Vazquez, M. Alfonso, B. Lanne, K. A. Karlsson, A. Carr, O. Barroso, L. E. Fernandez, E. Rengifo, M. E. Lanio, C. Alvarez, J. Zeuthen, R. Perez

Dept. of Tumor Cell Biology

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

86 Citas (Scopus)

Resumen

The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

Idioma original	Inglés
Páginas (desde-hasta)	551-556
Número de páginas	6
Publicación	Hybridoma
Volumen	14
N.º	6
DOI	https://doi.org/10.1089/hyb.1995.14.551
Estado	Publicada - 1995
Publicado de forma externa	Sí

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Vazquez, A. M., Alfonso, M., Lanne, B., Karlsson, K. A., Carr, A., Barroso, O., Fernandez, L. E., Rengifo, E., Lanio, M. E., Alvarez, C., Zeuthen, J., & Perez, R. (1995). Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids. Hybridoma, 14(6), 551-556. https://doi.org/10.1089/hyb.1995.14.551

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title = "Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids",

abstract = "The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.",

author = "Vazquez, \{A. M.\} and M. Alfonso and B. Lanne and Karlsson, \{K. A.\} and A. Carr and O. Barroso and Fernandez, \{L. E.\} and E. Rengifo and Lanio, \{M. E.\} and C. Alvarez and J. Zeuthen and R. Perez",

year = "1995",

doi = "10.1089/hyb.1995.14.551",

language = "English",

volume = "14",

pages = "551--556",

journal = "Hybridoma",

issn = "0272-457X",

number = "6",

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Vazquez, AM, Alfonso, M, Lanne, B, Karlsson, KA, Carr, A, Barroso, O, Fernandez, LE, Rengifo, E, Lanio, ME, Alvarez, C, Zeuthen, J & Perez, R 1995, 'Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids', Hybridoma, vol. 14, n.º 6, pp. 551-556. https://doi.org/10.1089/hyb.1995.14.551

TY - JOUR

T1 - Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

AU - Vazquez, A. M.

AU - Alfonso, M.

AU - Lanne, B.

AU - Karlsson, K. A.

AU - Carr, A.

AU - Barroso, O.

AU - Fernandez, L. E.

AU - Rengifo, E.

AU - Lanio, M. E.

AU - Alvarez, C.

AU - Zeuthen, J.

AU - Perez, R.

PY - 1995

Y1 - 1995

N2 - The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

AB - The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

UR - https://www.scopus.com/pages/publications/0029586061

U2 - 10.1089/hyb.1995.14.551

DO - 10.1089/hyb.1995.14.551

M3 - Article

C2 - 8770642

AN - SCOPUS:0029586061

SN - 0272-457X

VL - 14

SP - 551

EP - 556

JO - Hybridoma

JF - Hybridoma

IS - 6

ER -

Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

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