Electrochemical enzyme immunoassays on microchip platforms

J. Wang, A. Ibáñez, M. P. Chatrathi, A. Escarpa

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

168 Citas (Scopus)

Resumen

A microfluidic device for conducting electrochemical enzyme immunoassays is described. The new "lab-on-a-chip" protocol integrates precolumn reactions of alkaline phosphatase-labeled antibody (anti-mouse IgG) with the antigen (mouse IgG), followed by electrophoretic separation of the free antibody and antibody-antigen complex. The separation is followed by a postcolumn reaction of the enzyme tracer with the 4-aminophenyl phosphate substrate and a downstream amperometric detection of the liberated 4-aminophenol product. Factors influencing the reaction, separation, and detection processes were optimized, and the analytical performance was characterized. An applied field strength of 256 V/cm results in free antibody and antibody-antigen complex migration times of 125 and 340 s, respectively. A remarkably low detection limit of 2.5 × 10-16 g/mL (1.7 × 10-18 M) is obtained for the mouse IgG model analyte. Such combination of a complete integrated immunoassay, an attractive analytical performance, and the distinct miniaturization/portability advantages of electrochemical microsystems offers considerable promise for designing self-contained and disposable chips for decentralized clinical diagnostics or on-site environmental testing.

Idioma originalInglés
Páginas (desde-hasta)5323-5327
Número de páginas5
PublicaciónAnalytical Chemistry
Volumen73
N.º21
DOI
EstadoPublicada - 1 nov. 2001
Publicado de forma externa

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