Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells

Juan M. Lopez

Producción científica: Capítulo del libro/informe/acta de congresoCapítulorevisión exhaustiva

Resumen

Studies of intrinsically disordered proteins (IDPs) under physiological conditions by conventional NMR methods based on proton detection are severely limited by fast proton amide solvent exchange. Carbon detection has been proposed as a solution to the exchange problem but is hampered by low sensitivity. Here, we present a protocol combining proton–nitrogen cross-polarization and carbonyl detection to record high-resolution and high-sensitivity NMR spectra of IDPs under physiological conditions. The protocol describes a step-by-step method to register high-quality N-CO correlation spectrum of alpha-synuclein in E.coli bacterial cells at 37 °C.

Idioma originalInglés
Título de la publicación alojadaMethods in Molecular Biology
EditorialHumana Press Inc.
Páginas449-460
Número de páginas12
DOI
EstadoPublicada - 2023

Serie de la publicación

NombreMethods in Molecular Biology
Volumen2551
ISSN (versión impresa)1064-3745
ISSN (versión digital)1940-6029

Huella

Profundice en los temas de investigación de 'Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells'. En conjunto forman una huella única.

Citar esto