@inbook{6bab41ae0db54f67bedacb19b902fe08,
title = "Combined H-N Cross-Polarization and Carbonyl Detection NMR Spectroscopy Allow to Record High-Resolution, High-Sensitivity Spectra of Alpha-Synuclein in Bacterial Cells",
abstract = "Studies of intrinsically disordered proteins (IDPs) under physiological conditions by conventional NMR methods based on proton detection are severely limited by fast proton amide solvent exchange. Carbon detection has been proposed as a solution to the exchange problem but is hampered by low sensitivity. Here, we present a protocol combining proton–nitrogen cross-polarization and carbonyl detection to record high-resolution and high-sensitivity NMR spectra of IDPs under physiological conditions. The protocol describes a step-by-step method to register high-quality N-CO correlation spectrum of alpha-synuclein in E.coli bacterial cells at 37 °C.",
keywords = "Alpha-synuclein, Carbon detection NMR, In vivo NMR, In-cell NMR, Intrinsically disordered protein, J cross-polarization",
author = "Lopez, {Juan M.}",
note = "Publisher Copyright: {\textcopyright} 2023, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2597-2_28",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "449--460",
booktitle = "Methods in Molecular Biology",
}