Clinical evidences of GM3 (NeuGc) ganglioside expression in human breast cancer using the 14F7 monoclonal antibody labelled with 99mTc

Juan P. Oliva, Zodilina Valdés, Angel Casacó, Gilmara Pimentel, Joaquín González, Irene Álvarez, Martha Osorio, Milagros Velazco, Mariela Figueroa, Rosa Ortiz, Xiomara Escobar, Maiby Orozco, Julia Cruz, Sonia Franco, Mirtha Díaz, Lourdes Roque, Adriana Carr, Ana M. Vázquez, Cristina Mateos, María C. RubioRolando Pérez, Luis E. Fernández

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

50 Citas (Scopus)

Resumen

The relevance of certain gangliosides in tumour growth and metastatic dissemination has been well documented, reasons for considering these molecules as potential targets for cancer immunotherapy and diagnosis. GM3(NeuGc) ganglioside is particularly interesting due to its restrictive expression in normal human tissues according to immunohistochemical studies, using either polyclonal or monoclonal antibodies. But both immunohistochemical and biochemical methods have strongly suggested its over-expression in human breast tumours. Nevertheless, the lack of a direct evidence of this antigenic display in human breast cancer has kept the subject controversial. For the first time, we described herein the "in vivo" detection of GM3(NeuGc) ganglioside in human breast primary tumours using a radioimmunoscintigraphic technique with 14F7, a highly specific anti-GM3(NeuGc) ganglioside monoclonal antibody, labelled with 99mTc. In an open, prospective Phase I/II clinical trial, including women diagnosed in stage II breast cancer, the 14F7 monoclonal antibody accumulation in tumours at doses of 0.3 (n = 5), 1 (n = 5) and 3 mg (n = 4) was evaluated. Noteworthy, the immunoscintigraphic study showed antibody accumulation in 100% of patients' tumours for the 1 mg dose group. In turn, the radioimmunoconjugate injected at doses of 0.3 mg or 3 mg of the antibody, was uptaken by 60 and 33.3% of breast tumours, respectively. "In vivo" immune recognition of GM3(NeuGc) in breast tumours reinforces the value of this peculiar target for cancer immunotherapy.

Idioma originalInglés
Páginas (desde-hasta)115-121
Número de páginas7
PublicaciónBreast Cancer Research and Treatment
Volumen96
N.º2
DOI
EstadoPublicada - mar. 2006
Publicado de forma externa

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