Resumen
In order to investigate metabolic properties of single cells of freshwater algae (Haematococcus pluvialis), we implement matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) in combination with microspectroscopic mapping. Straightforward coupling of these two detection platforms was possible thanks to the self-aliquoting properties of micro-arrays for mass spectrometry (MAMS). Following Raman and fluorescence imaging, the isolated cells were covered with a MALDI matrix for targeted metabolic analysis by MALDI-MS. The three consecutive measurements carried out on the same cells yielded complementary information. Using this method, we were able to study the encystment of H. pluvialis-by monitoring the adenosine triphosphate (ATP) to adenosine diphosphate (ADP) ratio during the build-up of astaxanthin in the cells as well as the release of β-carotene, the precursor of astaxanthin, into the cytosol.
Idioma original | Inglés |
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Páginas (desde-hasta) | 6732-6736 |
Número de páginas | 5 |
Publicación | Analyst |
Volumen | 138 |
N.º | 22 |
DOI | |
Estado | Publicada - 21 nov. 2013 |
Publicado de forma externa | Sí |