A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors

A. Carr; A. Mullet; Z. Mazorra; A. M. Vázquez; M. Alfonso; C. Mesa; E. Rengifo; R. Pérez; L. E. Fernández

doi:10.1089/02724570050109639

A mouse IgG₁ monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors

A. Carr, A. Mullet, Z. Mazorra, A. M. Vázquez, M. Alfonso, C. Mesa, E. Rengifo, R. Pérez, L. E. Fernández

Center of Molecular Immunology Havana

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108 Citas (Scopus)

Resumen

14F7 murine monoclonal antibody (MAb) is an IgG1 immunoglobulin that is generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins and in the presence of Freund's adjuvants. 14F7 MAb binds specifically to GM3(NeuGc), whereas neither N-glycolyl or N-acetyl gangliosides, nor a sulfated glycolipid, are recognized as assessed by enzyme-linked immunosorbent assay or immunostaining on thin layer chromatograms. Immunohistochemical studies in fresh tumor tissues showed that 14F7 MAb strongly recognized in antigen expressed in human breast and melanoma tumors.

Idioma original	Inglés
Páginas (desde-hasta)	241-247
Número de páginas	7
Publicación	Hybridoma
Volumen	19
N.º	3
DOI	https://doi.org/10.1089/02724570050109639
Estado	Publicada - 2000
Publicado de forma externa	Sí

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title = "A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors",

abstract = "14F7 murine monoclonal antibody (MAb) is an IgG1 immunoglobulin that is generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins and in the presence of Freund's adjuvants. 14F7 MAb binds specifically to GM3(NeuGc), whereas neither N-glycolyl or N-acetyl gangliosides, nor a sulfated glycolipid, are recognized as assessed by enzyme-linked immunosorbent assay or immunostaining on thin layer chromatograms. Immunohistochemical studies in fresh tumor tissues showed that 14F7 MAb strongly recognized in antigen expressed in human breast and melanoma tumors.",

author = "A. Carr and A. Mullet and Z. Mazorra and V{\'a}zquez, {A. M.} and M. Alfonso and C. Mesa and E. Rengifo and R. P{\'e}rez and Fern{\'a}ndez, {L. E.}",

year = "2000",

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T1 - A mouse IgG1 monoclonal antibody specific for N-glycolyl GM3 ganglioside recognized breast and melanoma tumors

AU - Carr, A.

AU - Mullet, A.

AU - Mazorra, Z.

AU - Vázquez, A. M.

AU - Alfonso, M.

AU - Mesa, C.

AU - Rengifo, E.

AU - Pérez, R.

AU - Fernández, L. E.

PY - 2000

Y1 - 2000

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AB - 14F7 murine monoclonal antibody (MAb) is an IgG1 immunoglobulin that is generated by immunizing Balb/c mice with GM3(NeuGc) ganglioside hydrophobically conjugated with human very-low-density lipoproteins and in the presence of Freund's adjuvants. 14F7 MAb binds specifically to GM3(NeuGc), whereas neither N-glycolyl or N-acetyl gangliosides, nor a sulfated glycolipid, are recognized as assessed by enzyme-linked immunosorbent assay or immunostaining on thin layer chromatograms. Immunohistochemical studies in fresh tumor tissues showed that 14F7 MAb strongly recognized in antigen expressed in human breast and melanoma tumors.

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