Use of mass spectrometry coupled to chromatography (LC-MS/MS) for the identification of changes in the metabolism of the microorganisms Amycolaptosis sp.

Metabolomics provides valuable information on cellular function and response to external stimuli. We propose the combination of liquid chromatography (LC) coupled with mass spectrometry (MS) to perform this study. Liquid chromatography is an analytical tool that allows the separation of complex mixtures. At the same time, mass spectrometry (MS) provides high sensitivity with the added value of the structural information provided by knowledge of the molecular weight of the compound studied and its possible fragments. The union of both methods gives us the advantage of analyzing a large set of secondary metabolites. Metabolomic studies using mass spectrometry are so powerful that they can be performed at the single-cell level without derivatization. In comparative experiments, metabolomics applied to bacteria allows the identification of biomarkers capable of differentiating strains.

Identification of the metabolic changes of the microorganism Amycolatopsis sp. because of abiotic tress.

• Identify the metabolic pathway necessary for the microorganism Amycolatopsis sp. to overcome abiotic stress from heavy metals in the growth medium. • Compare the metabolic profile of the microorganism Amycolatopsis sp. with A. eurytherma (biologic control) under similar abiotic stress (due to heavy metals) to better understand the underlying biological process.

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publicación de resultados y tesis de doctorado en argentina

Investigacion basica

Disciplinario

ADMINISTRADO

LIMA - LIMA - SAN MIGUEL

• 76 — Medioambiente, recursos naturales y ecología

Ciencias naturales - Biología - Biología celular, Microbiología

PONTIFICIA UNIVERSIDAD CATÓLICA DEL PERÚ