Use of mass spectrometry coupled to chromatography (LC-MS/MS) for the identification of changes in the metabolism of the microorganisms Amycolaptosis sp

Acronym

P_LCMS-AMY

Consortium Coordinator

Silva Manco, Maria Julia

Start Date

August 2, 2024

End Date

November 8, 2024

Status

https://purl.org/pe-repo/concytec/estadoProyecto#concluido

Tipo de proyecto

https://purl.org/pe-repo/ocde/tipoProyecto#investigacionBasica

Description

Metabolomics provides valuable information on cellular function and response to external stimuli. We propose the combination of liquid chromatography (LC) coupled with mass spectrometry (MS) to perform this study. Liquid chromatography is an analytical tool that allows the separation of complex mixtures. At the same time, mass spectrometry (MS) provides high sensitivity with the added value of the structural information provided by knowledge of the molecular weight of the compound studied and its possible fragments. The union of both methods gives us the advantage of analyzing a large set of secondary metabolites. Metabolomic studies using mass spectrometry are so powerful that they can be performed at the single-cell level without derivatization. In comparative experiments, metabolomics applied to bacteria allows the identification of biomarkers capable of differentiating strains.

Keywords

Espectrometría de masas

;

Metabolómica microbiana

;

Amycolatopsis

;

Cromatografía líquida

Área de conocimiento

Natural sciences

Campo OCDE

https://purl.org/pe-repo/ocde/ford#1.06.13