Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA

Ch Rodríguez-Tanty; R. Pérez; J. Miranda; H. Vélez-Castro; A. Rosado; A. Macías; V. Vidal-Alles; M. J. Araña; N. Pérez Souto; D. Higginson-Clarke; A. M. Riverón

doi:10.1081/NCN-100105240

Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA

Ch Rodríguez-Tanty, R. Pérez, J. Miranda, H. Vélez-Castro, A. Rosado, A. Macías, V. Vidal-Alles, M. J. Araña, N. Pérez Souto, D. Higginson-Clarke, A. M. Riverón

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3 Scopus citations

Abstract

We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by ¹H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.

Original language	English
Pages (from-to)	1449-1461
Number of pages	13
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	20
Issue number	8
DOIs	https://doi.org/10.1081/NCN-100105240
State	Published - 2001
Externally published	Yes

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Rodríguez-Tanty, C., Pérez, R., Miranda, J., Vélez-Castro, H., Rosado, A., Macías, A., Vidal-Alles, V., Araña, M. J., Souto, N. P., Higginson-Clarke, D., & Riverón, A. M. (2001). Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA. Nucleosides, Nucleotides and Nucleic Acids, 20(8), 1449-1461. https://doi.org/10.1081/NCN-100105240

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title = "Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA",

abstract = "We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.",

author = "Ch Rodr{\'i}guez-Tanty and R. P{\'e}rez and J. Miranda and H. V{\'e}lez-Castro and A. Rosado and A. Mac{\'i}as and V. Vidal-Alles and Ara{\~n}a, {M. J.} and Souto, {N. P{\'e}rez} and D. Higginson-Clarke and River{\'o}n, {A. M.}",

year = "2001",

doi = "10.1081/NCN-100105240",

language = "English",

volume = "20",

pages = "1449--1461",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

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Rodríguez-Tanty, C, Pérez, R, Miranda, J, Vélez-Castro, H, Rosado, A, Macías, A, Vidal-Alles, V, Araña, MJ, Souto, NP, Higginson-Clarke, D & Riverón, AM 2001, 'Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA', Nucleosides, Nucleotides and Nucleic Acids, vol. 20, no. 8, pp. 1449-1461. https://doi.org/10.1081/NCN-100105240

TY - JOUR

T1 - Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA

AU - Rodríguez-Tanty, Ch

AU - Pérez, R.

AU - Miranda, J.

AU - Vélez-Castro, H.

AU - Rosado, A.

AU - Macías, A.

AU - Vidal-Alles, V.

AU - Araña, M. J.

AU - Souto, N. Pérez

AU - Higginson-Clarke, D.

AU - Riverón, A. M.

PY - 2001

Y1 - 2001

N2 - We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.

AB - We report the synthesis of the triphosphate of 5-methyl 4-N-[6-(p-bromobenzamido)hex-l-yl]-2′-O-deoxycytidine 3A. We also analyzed the formation of intramolecular H-bonds of 5-methyl 4-N-(n-[6-(p-bromobenzamido) caproyl amino]alk-l-yl)-2′-deoxycytidine compounds, and confirmed their presence by 1H-NMR studies. In vitro DNA labeling with modified nucleotides is preliminarily evaluated.

UR - http://www.scopus.com/inward/record.url?scp=0034866333&partnerID=8YFLogxK

U2 - 10.1081/NCN-100105240

DO - 10.1081/NCN-100105240

M3 - Article

C2 - 11554538

AN - SCOPUS:0034866333

SN - 1525-7770

VL - 20

SP - 1449

EP - 1461

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 8

ER -

Synthesis of a new 5′-O-triphosphate analog of 5-methyl 2′-O-deoxycytidine. Preliminary in vitro labeling for non-radioactive detection of DNA

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