Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Mario Culebras; C. Grande; F. G. Torres; Omar P. Troncoso; Clara M. Gomez; M. Carmen Bañó

Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Mario Culebras, C. Grande, F. G. Torres, Omar P. Troncoso, Clara M. Gomez, M. Carmen Bañó

Sección de Ingeniería Mecánica

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

Original language	Spanish
Pages (from-to)	411-415
Number of pages	5
Journal	International Journal of Polymeric Materials and Polymeric Biomaterials
Volume	64
State	Published - 1 Jan 2015

Cite this

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title = "Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine",

abstract = "Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.",

author = "Mario Culebras and C. Grande and Torres, {F. G.} and Troncoso, {Omar P.} and Gomez, {Clara M.} and Ba{\~n}{\'o}, {M. Carmen}",

year = "2015",

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T1 - Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

AU - Culebras, Mario

AU - Grande, C.

AU - Torres, F. G.

AU - Troncoso, Omar P.

AU - Gomez, Clara M.

AU - Bañó, M. Carmen

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

AB - Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

M3 - Artículo

SN - 2574-0881

VL - 64

SP - 411

EP - 415

JO - International Journal of Polymeric Materials and Polymeric Biomaterials

JF - International Journal of Polymeric Materials and Polymeric Biomaterials

ER -