Optimization of Cell Growth on Bacterial Cellulose by Adsorption of Collagen and Poly-L-Lysine

Mario Culebras, Cristian J. Grande, Fernando G. Torres, Omar P. Troncoso, Clara M. Gomez, M. Carmen Bañó

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Poly-L-lysine and collagen were separately added to bacterial cellulose (BC) nanofibers. The ionic surface charge had been previously modified in order to promote the adsorption of poly-L-lysine and collagen. Cell adhesion of Chinese hamster ovary (CHO) cells on BC surfaces was confirmed by removing unattached cells from the BC substrates. Cell viability was calculated and it was determined that both poly-L-lysine-BC and collagen-BC substrates are viable for cell growth. The results showed that the cell viability in poly-L-lysine modified BC substrate is similar to the one observed in polystyrene tissue culture plates.

Original languageEnglish
Pages (from-to)411-415
Number of pages5
JournalInternational Journal of Polymeric Materials and Polymeric Biomaterials
Volume64
Issue number8
DOIs
StatePublished - 12 Sep 2015

Keywords

  • Bacterial cellulose
  • cell growth
  • collagen
  • poly-L-lysine
  • tissue engeneering

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