Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

A. M. Vazquez; M. Alfonso; B. Lanne; K. A. Karlsson; A. Carr; O. Barroso; L. E. Fernandez; E. Rengifo; M. E. Lanio; C. Alvarez; J. Zeuthen; R. Perez

doi:10.1089/hyb.1995.14.551

Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

A. M. Vazquez
, M. Alfonso
, B. Lanne
, K. A. Karlsson
, A. Carr
, O. Barroso
, L. E. Fernandez
, E. Rengifo
, M. E. Lanio
, C. Alvarez
, J. Zeuthen
, R. Perez

Dept. of Tumor Cell Biology

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

Original language	English
Pages (from-to)	551-556
Number of pages	6
Journal	Hybridoma
Volume	14
Issue number	6
DOIs	https://doi.org/10.1089/hyb.1995.14.551
State	Published - 1995
Externally published	Yes

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Vazquez, A. M., Alfonso, M., Lanne, B., Karlsson, K. A., Carr, A., Barroso, O., Fernandez, L. E., Rengifo, E., Lanio, M. E., Alvarez, C., Zeuthen, J., & Perez, R. (1995). Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids. Hybridoma, 14(6), 551-556. https://doi.org/10.1089/hyb.1995.14.551

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title = "Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids",

abstract = "The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.",

author = "Vazquez, \{A. M.\} and M. Alfonso and B. Lanne and Karlsson, \{K. A.\} and A. Carr and O. Barroso and Fernandez, \{L. E.\} and E. Rengifo and Lanio, \{M. E.\} and C. Alvarez and J. Zeuthen and R. Perez",

year = "1995",

doi = "10.1089/hyb.1995.14.551",

language = "English",

volume = "14",

pages = "551--556",

journal = "Hybridoma",

issn = "0272-457X",

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}

Vazquez, AM, Alfonso, M, Lanne, B, Karlsson, KA, Carr, A, Barroso, O, Fernandez, LE, Rengifo, E, Lanio, ME, Alvarez, C, Zeuthen, J & Perez, R 1995, 'Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids', Hybridoma, vol. 14, no. 6, pp. 551-556. https://doi.org/10.1089/hyb.1995.14.551

TY - JOUR

T1 - Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

AU - Vazquez, A. M.

AU - Alfonso, M.

AU - Lanne, B.

AU - Karlsson, K. A.

AU - Carr, A.

AU - Barroso, O.

AU - Fernandez, L. E.

AU - Rengifo, E.

AU - Lanio, M. E.

AU - Alvarez, C.

AU - Zeuthen, J.

AU - Perez, R.

PY - 1995

Y1 - 1995

N2 - The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

AB - The P3 murine monoclonal antibody (MAb) was generated by immunizing BALB/c mice with NeuGcGM3 included into liposomes. The specificity of this MAb was defined by an enzyme-linked immunosorbent assay and immunostaining on thin- layer chromatograms. P3 MAb binds to NeuGc-containing gangliosides and was shown also to react with sulfated glycolipids. A preliminary immunohistochemical study showed that the P3 MAb was able to recognize antigens expressed in human breast tumors.

UR - https://www.scopus.com/pages/publications/0029586061

U2 - 10.1089/hyb.1995.14.551

DO - 10.1089/hyb.1995.14.551

M3 - Article

C2 - 8770642

AN - SCOPUS:0029586061

SN - 0272-457X

VL - 14

SP - 551

EP - 556

JO - Hybridoma

JF - Hybridoma

IS - 6

ER -

Generation of a murine monoclonal antibody specific for N- glycolylneuraminic acid-containing gangliosides that also recognizes sulfated glycolipids

Abstract

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