Gangliosides, Ab1 and Ab2 antibodies. IV. Dominance of VH domain in the induction of anti-idiotypic antibodies by gene gun immunization

Alejandro López-Requena, Cristina Mateo de Acosta, Marco Bestagno, Ana María Vázquez, Rolando Pérez, Oscar R. Burrone

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The heavy chain of anti-N-glycolyl-ganglioside P3 mAb plays the main role in its binding properties. At least one hybrid idiotype consisting on the P3 VH and an unrelated VL domain retains antigen recognition. Moreover, the unusual immunogenic properties of P3 idiotype could be modified by single mutations of H-CDR residues. Here, we show that DNA gene gun immunization with the P3 VH combined with an unrelated VL domain or with itself (VH dimer, VHD) is enough for inducing anti-idiotypic antibodies, independently of antigen recognition by the resulting molecule. The scFv fragment of P3 mAb was also able to induce an anti-idiotypic response. For both the P3 and the P3 anti-idiotypic 1E10 mAbs, heavy chains dominate the induction of antibodies against the respective idiotypes.

Original languageEnglish
Pages (from-to)3070-3075
Number of pages6
JournalMolecular Immunology
Volume44
Issue number11
DOIs
StatePublished - Apr 2007
Externally publishedYes

Keywords

  • Anti-idiotypic antibodies
  • Hybrid idiotype
  • scFv
  • VH dimer

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