Characterization of the colorectal antigen IOR-C2

A. M. Vazquez; B. Tormo; A. Velandia; R. Giscombe; I. Ansotegui; M. Jeddi-Tehrani; R. Perez; H. Mellstedt; P. Biberfeld

doi:10.1089/hyb.1992.11.245

Characterization of the colorectal antigen IOR-C2

A. M. Vazquez, B. Tormo, A. Velandia, R. Giscombe, I. Ansotegui, M. Jeddi-Tehrani, R. Perez, H. Mellstedt, P. Biberfeld

Karolinska Universitetssjukhuset

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.

Original language	English
Pages (from-to)	245-256
Number of pages	12
Journal	Hybridoma
Volume	11
Issue number	2
DOIs	https://doi.org/10.1089/hyb.1992.11.245
State	Published - 1992
Externally published	Yes

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title = "Characterization of the colorectal antigen IOR-C2",

abstract = "A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.",

author = "Vazquez, {A. M.} and B. Tormo and A. Velandia and R. Giscombe and I. Ansotegui and M. Jeddi-Tehrani and R. Perez and H. Mellstedt and P. Biberfeld",

year = "1992",

doi = "10.1089/hyb.1992.11.245",

language = "English",

volume = "11",

pages = "245--256",

journal = "Hybridoma",

issn = "0272-457X",

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TY - JOUR

T1 - Characterization of the colorectal antigen IOR-C2

AU - Vazquez, A. M.

AU - Tormo, B.

AU - Velandia, A.

AU - Giscombe, R.

AU - Ansotegui, I.

AU - Jeddi-Tehrani, M.

AU - Perez, R.

AU - Mellstedt, H.

AU - Biberfeld, P.

PY - 1992

Y1 - 1992

N2 - A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.

AB - A colorectal antigen (IOR-C2) was characterized by a monoclonal antibody produced against the colon cancer cell line SW1116. By immunohistochemical staining the antigen was abundant and strongly expressed in epithelium of normal colon whereas colorectal carcinomas showed a more variable and heterogenous reactivity to the antibody (IOR-C2). Radioimmunoprecipitates of SW1116 cell homogenates showed a 160-200 kD band in SDS gels. Physicochemical characterization indicate that at least two IOR-C2 reactive sites are present on the antigen tested and that it is mainly an 0-linked glycoprotein carbohydrate chain which can also be N-linked to the protein. The expression of IOR-C2 mimics that of the colon associated antigen (CAA) and NCC-CO-450 antigen but is distinct from these with regard to its expression in carcinomas as well as its physicochemical characteristics.

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U2 - 10.1089/hyb.1992.11.245

DO - 10.1089/hyb.1992.11.245

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VL - 11

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EP - 256

JO - Hybridoma

JF - Hybridoma

IS - 2

ER -

Characterization of the colorectal antigen IOR-C2

Abstract

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