TY - GEN
T1 - An analysis-synthesis approach for neurosphere modelisation under phase-contrast microscopy
AU - Rigaud, Stephane
AU - Huang, Chao Hui
AU - Ahmed, Sohail
AU - Lim, Joo Hwee
AU - Racoceanu, Daniel
PY - 2013
Y1 - 2013
N2 - The study of stem cells is one of the most important biomedical research. Understanding their development could allow multiple applications in regenerative medicine. For this purpose, automated solutions for the observation of stem cell development process are needed. This study introduces an on-line analysis method for the modelling of neurosphere evolution during the early time of their development under phase contrast microscopy. From the corresponding phase contrast time-lapse sequences, we extract information from the neurosphere using a combination of phase contrast physics deconvolution and curve detection for locate the cells inside the neurosphere. Then, based on prior biological knowledge, we generate possible and optimal 3-dimensional configuration using 2D to 3D registration methods and evolutionary optimisation algorithm.
AB - The study of stem cells is one of the most important biomedical research. Understanding their development could allow multiple applications in regenerative medicine. For this purpose, automated solutions for the observation of stem cell development process are needed. This study introduces an on-line analysis method for the modelling of neurosphere evolution during the early time of their development under phase contrast microscopy. From the corresponding phase contrast time-lapse sequences, we extract information from the neurosphere using a combination of phase contrast physics deconvolution and curve detection for locate the cells inside the neurosphere. Then, based on prior biological knowledge, we generate possible and optimal 3-dimensional configuration using 2D to 3D registration methods and evolutionary optimisation algorithm.
UR - https://www.scopus.com/pages/publications/84886569710
U2 - 10.1109/EMBC.2013.6610419
DO - 10.1109/EMBC.2013.6610419
M3 - Conference contribution
C2 - 24110606
AN - SCOPUS:84886569710
SN - 9781457702167
T3 - Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
SP - 3989
EP - 3992
BT - 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
T2 - 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Y2 - 3 July 2013 through 7 July 2013
ER -